Purpose
This kit allows for the determination of HDL-C concentrations in Human serum, blood
plasma, and other biological fluids.
Principle of the assay
The kit assay Human HDL-C level in the sample
，
use Purified Human HDL-C antibody to
coat microtiter plate wells, make solid-phase antibody, then add HDL-C to wells, Combined
HDL-C antibody which With HRP labeled
，
become antibody-antigen-
enzyme-antibody
complex, after washing Completely, Add TMB substrate
solution, TMB substrate becomes blue
color At HRPenzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid
solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm. The
concentration of
HDL
C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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